Journal: Scientific Reports

Article Title: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

doi: 10.1038/srep24865

Figure Lengend Snippet: Characteristics of HBV replication in HBVs-mut and HBV-s-rec strains.

Article Snippet: The following antibodies were used: anti-HBV core (HBcAg) (16), HBsAg antibody (Aviva Systems Biology, San Diego, CA, USA) and Gapdh (Cell Signaling, Danvers, MA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

doi: 10.1038/srep24865

Figure Lengend Snippet: Two-month-old transgenic HBV (HBV-s-mut) mice were given 200 μl of small interfering RNA (siRNA) (4 μg/g body weight) via tail vein injection. Mice were put to death 2 days or 10 days after injection, and liver and blood samples were collected. RNA was extracted from liver tissue, and changes in HBV mRNA expression were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) ( A ). Copy numbers were normalized to 100,000 copies of glyceraldehyde 3-phosphate dehydrogenase ( Gapdh ) (mean ± SEM). Serum was prepared, and hepatitis B excretory antigen (HBeAg) levels were measured by chemiluminescent microparticle immunoassay (CMIA) (mean ± SEM) ( B ). Proteins were extracted from liver tissue, and HBcAg was detected by western blot analysis ( C ). Densitometric analysis was performed ( D ). Liver tissue was fixed, embedded in paraffin, and sectioned; it was then stained with HBcAg/hematoxylin. Images (20x optical magnification) show cells from wild type (WT) mice, from HBV-s-mut mice given non-template control siRNA (siNC), and from HBV-s-mut mice given HBxAg-targeting siRNA (siHBV) ( E ). DNA was extracted from liver tissue, and changes in DNA replication of HBV were determined by Southern blot analysis ( F ). Group size n = 3 ( A , C/D ) or exemplarily n = 2 ( B , F ) animals; *p < 0.05, ***p < 0.001; d, day; w/o, without treatment.

Article Snippet: The following antibodies were used: anti-HBV core (HBcAg) (16), HBsAg antibody (Aviva Systems Biology, San Diego, CA, USA) and Gapdh (Cell Signaling, Danvers, MA, USA).

Techniques: Transgenic Assay, Small Interfering RNA, Injection, Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Staining, Control, Southern Blot

Journal: Scientific Reports

Article Title: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

doi: 10.1038/srep24865

Figure Lengend Snippet: Transgenic HBV (HBV-s-mut) mice were crossbred with Toll-like receptor 3-deficient (Tlr3−/−) mice. Two-month-old HBV-negative littermates (WT), Tlr3−/− mice, HBV-s-mut mice, and HBV-s-mut/Tlr3−/− mice were put to death. RNA was extracted from liver tissue and changes in gene expression of Ifnb1 ( A ), Isg15 ( B ), Ifit1 ( C ), Tlr3 ( G ) and HBV mRNA expression ( D ) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Copy numbers were normalized to 100,000 copies of Gapdh (mean ± SEM). DNA was extracted from liver tissue, and changes in HBV DNA were quantified by PCR ( E ). Proteins were extracted from liver tissue, HBcAg was detected by western blot analysis and densitometric analysis was performed ( F ). Group size n = 8 ( A – D ), n = 4 ( E , G ) and n = 3 ( F ) animals; *p < 0.05, **p < 0.01, ***p < 0.001; HBV-s-mut, Tg1.4HBV-S-Mut3; WT, wild type.

Article Snippet: The following antibodies were used: anti-HBV core (HBcAg) (16), HBsAg antibody (Aviva Systems Biology, San Diego, CA, USA) and Gapdh (Cell Signaling, Danvers, MA, USA).

Techniques: Transgenic Assay, Gene Expression, Expressing, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: Scientific Reports

Article Title: Hepatitis B virus genome replication triggers toll-like receptor 3-dependent interferon responses in the absence of hepatitis B surface antigen

doi: 10.1038/srep24865

Figure Lengend Snippet: Two-month-old male transgenic HBV (HBV-s-mut) mice and their wild type (WT) littermates were given Toll-like receptor 3 (Tlr3) ligand polyinosinic-polycytidylic acid (Poly[I:C]; 4 μg/g body weight) via tail vein injection. Mice were put to death 6 h or 24 h after injection. RNA was extracted, and changes in gene expression of Ifnb1 ( A ), Isg15 ( B ), and HBV ( C ) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Copy numbers were normalized to 100,000 copies of Gapdh (mean ± SEM). DNA was extracted from liver tissue, and changes in HBV DNA were quantified by PCR ( D ). Proteins were extracted from liver tissue, HBcAg was detected by western blot analysis and densitometric analysis was performed ( E ). Group size n = 3 animals; *p < 0.05, **p < 0.01, ***p < 0.001; HBV-s-mut, Tg1.4HBV-S-Mut3; w/o, without treatment.

Article Snippet: The following antibodies were used: anti-HBV core (HBcAg) (16), HBsAg antibody (Aviva Systems Biology, San Diego, CA, USA) and Gapdh (Cell Signaling, Danvers, MA, USA).

Techniques: Transgenic Assay, Injection, Gene Expression, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz

Article Title: Sicherheit von Blut und Blutprodukten: Testmethoden zum Nachweis der Hepatitisviren B, C und E

doi: 10.1007/s00103-021-03480-0

Figure Lengend Snippet:

Article Snippet: Anti-HBc , Monolisa Anti-HBc Plus , Bio-Rad , Indirekt (Anti-Human-IgG, -IgM) , ELISA.

Techniques: Marker, Multiplex Assay, Diagnostic Assay, Virus, Enzyme-linked Immunosorbent Assay, Sandwich ELISA

Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz

Article Title: Sicherheit von Blut und Blutprodukten: Testmethoden zum Nachweis der Hepatitisviren B, C und E

doi: 10.1007/s00103-021-03480-0

Figure Lengend Snippet:

Article Snippet: Anti-HBc , Advia Centaur HBc Total 2 (HBcT2) , Siemens Healthcare Diagnostics , Sandwich , CLIA.

Techniques: Marker

Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz

Article Title: Sicherheit von Blut und Blutprodukten: Testmethoden zum Nachweis der Hepatitisviren B, C und E

doi: 10.1007/s00103-021-03480-0

Figure Lengend Snippet:

Article Snippet: Anti-HBc , Advia Centaur HBc Total 2 (HBcT2) , Siemens Healthcare Diagnostics , Sandwich , CLIA.

Techniques: Marker, Multiplex Assay, Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Sandwich ELISA

Journal: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz

Article Title: Sicherheit von Blut und Blutprodukten: Testmethoden zum Nachweis der Hepatitisviren B, C und E

doi: 10.1007/s00103-021-03480-0

Figure Lengend Snippet:

Article Snippet: Anti-HBc , Advia Centaur HBc Total 2 (HBcT2) , Siemens Healthcare Diagnostics , Sandwich , CLIA.

Techniques: